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KMID : 0545120170270122104
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 12 p.2104 ~ p.2111
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Production of Bacterial Quorum Sensing Antagonists, Caffeoyl- and Feruloyl-HSL, by an Artificial Biosynthetic Pathway
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Kang Sun-Young
Kim Bo-Min
Heo Kyung-Taek
Jang Jae-Hyuk
Kim Won-Gon
Hong Young-Soo
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Abstract
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A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.
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KEYWORD
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Artificial biosynthesis, phenylacetyl homoserine lactone, caffeoyl-HSL, feruloyl-HSL
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